Fyrestar generates an ensemble of inter-converting states.  The solution properties each molecule exhibits is the energy weighted contribution of all microstates in the ensemble.  Fyrestar captures the structural and energetic features of the component microstates in the ensemble to accurately predict the macroscopic behavior of proteins.  The graph above illustrates the predictive power of Fyrestar.  The experimental pH dependence of proton binding is predicted by Fyrestar with considerable precision.